平菇子實體分化發(fā)育相關(guān)基因片段的克隆

Cloning of Differentiation and Development Related Gene Fragm ents in Pleurotus ostreatus

馬愛民　關(guān)海山　

摘　要：采用RAP-PCR差異顯示技術(shù)對平菇菌絲體及原基、菇蕾和成熟子實體進行研究,獲得了在原基階段差異表達的3條cDNA片段POD1、POD2與POD3,經(jīng)過回收、重擴增、克隆與鑒定后進行測序分析,初步的結(jié)果表明3條差異片段的長度分別為745bp、482bp與477bp.同時,國際聯(lián)網(wǎng)檢索查明片段POD3為HSP70基因片段.

關(guān)鍵詞：平菇; 差異顯示; RAP?PCR; 分化發(fā)育相關(guān)基因; HSP70

分類號：Q344+.3; S646.1+4　文獻標(biāo)識碼：A

基金項目：國家自然科學(xué)基金資助項目(39770530)

作者簡介：馬愛民,男,1965年生,碩士,副教授.

作者單位：馬愛民（華中農(nóng)業(yè)大學(xué)植物保護系武漢 430070）　

　　　　　關(guān)海山（香港中文大學(xué)生物系香港）　

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華中農(nóng)業(yè)大學(xué)學(xué)報

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2001 Vol.20 No.4 P.299-302