Assessment The Genetic Diversity of Auricularia Strains by Two PCR-Based Typing Methods

Abstract：Two PCR-based methods, enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and randomly amplified polymorphic DNA (RAPD), were adopted for differentiating Auricularia strains. Taken the similarity coefficient as 75%, 29 strains of three Auricularia species were grouped into 6 and 9 clusters by RAPD and ERIC, respectively. The dendrogram from ERIC exhibited two distinct parts, one representing A. auricula and the other A. polytricha, but the dendrogram from RAPD failed to clearly distinguish between these two species. However, both methods similarly revealed high homology between A. fuscosuccinea and A. auricula. The homology relationships among the three species obtained from ERIC were validated by Southern hybridization. The analyses showed that RAPD is able to differentiate mainly at the species level, while ERIC is effective at the strain level and therefore more consistent with cultivation characteristics. The results indicate that the method of ERIC-PCR is more rapid and reliable than RAPD, and may substitute for RAPD in research related to the genetic identification and genetic diversity in Auricularia.

Keywords：Auricularia, ERIC-PCR, RAPD analysis, Genetic diversity

兩種PCR方法對木耳屬菌株的遺傳多樣性評價

溫亞麗　 曹暉　 潘迎捷　

摘　要：應(yīng)用ERIC和RAPD兩種PCR方法對木耳屬3種29個菌株進行遺傳鑒別,其中ERIC方法是首次運用于食用菌的研究領(lǐng)域. 在相似系數(shù)75%的水平上, ERIC和RAPD分別將供試菌株分為9組和6組. 由ERIC所得的聚類圖可將黑木耳和毛木耳兩個種區(qū)分開, 而RAPD則不能完全區(qū)分兩個種,但兩種方法得到了一個相似的結(jié)果, 即琥珀木耳與黑木耳的親緣關(guān)系極其相近. Southern雜交實驗進一步證明了ERIC所得到的29個菌株的同源性關(guān)系. 分析表明, RAPD方法主要在種的水平上進行鑒別, 而ERIC則可以在菌株水平上進行鑒別, 結(jié)果與菌株栽培性狀更為一致.研究結(jié)果表明ERIC-PCR是一種比RAPD更快捷可靠的分子標記方法, 可以替代RAPD應(yīng)用于木耳屬的遺傳多樣性及遺傳分類的研究.

關(guān)鍵詞：木耳屬, ERIC-PCR, RAPD, 遺傳多樣性

CLC Number：Q789 　Document ID：A

Article ID：0001-6209(2004)06-0810-06

Foundation Item：上海市農(nóng)業(yè)科學(xué)院發(fā)展基金項目

Author Resume：溫亞麗(1980-),女,安徽界首人,碩士研究生,主要從事木耳屬種質(zhì)資源遺傳鑒定方面的研究.曹暉,通訊作者.Tel:86-21-62208660轉(zhuǎn)3222;Fax:86-21-62201337;E-mail: syja5@saas.sh.cn

Author Unit：溫亞麗（南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院微生物系,南京,210095;上海市農(nóng)業(yè)科學(xué)院食用菌研究所,農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點開放實驗室,上海,201106）　

曹暉（上海市農(nóng)業(yè)科學(xué)院食用菌研究所,農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點開放實驗室,上海,201106）　

潘迎捷（上海市農(nóng)業(yè)科學(xué)院食用菌研究所,農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室,上海市農(nóng)業(yè)遺傳育種重點開放實驗室,上海,201106）　

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Manuscript Received：2004年3月8日

Manuscript Revised：2004年5月8日

Published：2004年12月1日