中國栽培白靈側(cè)耳的RAPD和IGS分析

RAPD AND IGS ANALYSIS OF PLEUROTUS NEBRODENSIS CULTIVARS IN CHINA

張金霞　黃晨陽　張瑞穎　管桂萍　

摘　要：以RAPD和IGS為分子標(biāo)記,研究了中國栽培白靈側(cè)耳Pleurotus nebrodensis菌株的遺傳多樣性,RAPD結(jié)果表明,供試菌株間存在多態(tài)性;對供試菌株的IGS分析表明,白靈側(cè)耳P.nebrodensis的IGSl區(qū)域較保守,菌株間沒有多態(tài)性;IGS2區(qū)域進(jìn)化較快,菌株間具豐富的多態(tài)性.IGS2-RFLP可應(yīng)用于白靈側(cè)耳的菌株鑒定鑒別,較RAPD更穩(wěn)定,更具可操作性.

關(guān)鍵詞：遺傳多樣性,菌株多態(tài)性,IGS2-RFLP

分類號：Q939.5 　文獻(xiàn)標(biāo)識碼：A

文章編號：1672-6472(2004)04-0514-0519

基金項(xiàng)目：引進(jìn)國際先進(jìn)農(nóng)業(yè)科學(xué)技術(shù)("948"計(jì)劃)項(xiàng)目(2004-Z21)

作者單位：張金霞（中國農(nóng)業(yè)科學(xué)院土壤肥料研究所,北京,100081）黃晨陽（中國農(nóng)業(yè)科學(xué)院土壤肥料研究所,北京,100081）張瑞穎（中國農(nóng)業(yè)科學(xué)院土壤肥料研究所,北京,100081）管桂萍（中國農(nóng)業(yè)科學(xué)院土壤肥料研究所,北京,100081）　

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收稿日期：2004年5月24日

修稿日期：2004年7月1日

出版日期：2004年11月22日

菌物學(xué)報

MYCOSYSTEMA

2004 Vol.23 No.4 P.514-519