銀耳的超聲波介導(dǎo)轉(zhuǎn)化
Transformation of Tremella fuciformis by Using Ultrasonic Mediation
謝寶貴 饒永斌 鄭金貴
摘 要：以銀耳(Tremellafuciformis)芽孢為實(shí)驗(yàn)材料,正交實(shí)驗(yàn)研究超聲波處理時(shí)間、間歇時(shí)間及功率對(duì)轉(zhuǎn)化率的影響.實(shí)驗(yàn)結(jié)果表明:超聲波處理時(shí)間與間歇時(shí)間對(duì)質(zhì)粒DNA和芽孢轉(zhuǎn)化率都有顯著影響;實(shí)驗(yàn)中質(zhì)粒DNA轉(zhuǎn)化率最高達(dá)到754.7個(gè)轉(zhuǎn)化子/μg DNA,銀耳芽孢轉(zhuǎn)化率達(dá)到1.28‰;轉(zhuǎn)化子的潮霉素抗性可達(dá)到200μg/mL,GUS檢測(cè)陽(yáng)性.
關(guān)鍵詞：銀耳;超聲波介導(dǎo)轉(zhuǎn)化;正交實(shí)驗(yàn)
基金項(xiàng)目：福建省科技項(xiàng)目(No.2001Z010)基金資助.
作者簡(jiǎn)介：謝寶貴:男,1962年生,博士,副教授.E-mail:＜xbg@pub2.fz.fj.cn＞.鄭金貴:通訊作者.Author for correspondence.E-mail:＜jinggui@fjau.edu.cn＞.
作者單位：謝寶貴（福建農(nóng)林大學(xué)生命科學(xué)學(xué)院,福州,350002;福建農(nóng)林大學(xué)菌物研究中心,福州,350002）
饒永斌（福建農(nóng)林大學(xué)生命科學(xué)學(xué)院,福州,350002;福建農(nóng)林大學(xué)菌物研究中心,福州,350002）
鄭金貴（福建農(nóng)林大學(xué)農(nóng)產(chǎn)品品質(zhì)研究所,福州,350002）
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農(nóng)業(yè)生物技術(shù)學(xué)報(bào)
JOURNAL OF AGRICULTURAL BIOTECHNOLOGY
2005 Vol.13 No.1 P.42-45
