古尼蟲(chóng)草無(wú)性型的分子鑒別 張澤文　 傅嵐　 陳作紅　 摘　要：采用PCR技術(shù),以rDNA的ITS區(qū)為分子指標(biāo),對(duì)古尼蟲(chóng)草Cordyceps gunnii的有性和無(wú)性階段進(jìn)行比較分析,從分子水平上證明古尼蟲(chóng)草的無(wú)性階段是古尼擬青霉Paecilomycesgunnii. 關(guān)鍵詞：古尼擬青霉;rDNAITS區(qū) 分類號(hào)：Q939.5　文獻(xiàn)標(biāo)識(shí)碼：A 文章編號(hào)：1672-6472(2005)03-0344-0348 MOLECULAR IDENTIFICATION OF THE ANAMORPH STAGE OF CORDYCEPS GUNNII ZHANG Ze-Wen　 FU Lan　 CHEN Zuo-Hong　 基金項(xiàng)目：湖南省教育廳青年基金資助項(xiàng)目(0lB010) 作者簡(jiǎn)介：陳作紅,通訊作者E-Mail:chenzuohong@263.net 作者單位：張澤文（湖南師范大學(xué)生命科學(xué)學(xué)院,長(zhǎng)沙,410081）　 　　　　　傅嵐（湖南師范大學(xué)生命科學(xué)學(xué)院,長(zhǎng)沙,410081）　 　　　　　陳作紅（湖南師范大學(xué)生命科學(xué)學(xué)院,長(zhǎng)沙,410081）　 參考文獻(xiàn)： [1]Liang Z Q, 1983. A description of Cordyceps gunnii in China. Acta Mycologica Sinica, 2 (4): 258～259 [2]Liang Z Q, 1985. Isolation and identification of the conidial stage of cordyceps gunnii. Acta Mycologica Sinica, 4 (3): 162～166 [3]Liu A Y, Liang Z Q, Liu Z Y, 1997. Determma tion of two species of Cordyceps by ascosporal microcycle condiation. Mycosystema,16 (4): 297～299 [4]Sambrook J, Russell D W, 2001. Molecular Cloning:A Laboratory Manual (3rd ed.). Cold Spring Harbor Laboratory Press. [5]Liang Z Q, 2001. Current situation and ponderation of Cordyceps Fr. research and exploitation in China. Acta Edulis Fungi, 8 (2): 53～62 [6]Li Z Z, Huang B, Li C R, Fan M Z, 2000. Molecular evidence for the asexual stage of Cordyceps sinensis (Berk.) Sacc. Mycosysterna,19 (1): 60～64 [7]Zhao J, Wang N, Chen Y Q, Li T H, Qu L H, 1999. Molecular identification for the asexual stage of Cordyceps sinensis. Acta Scientiarum Naturalium Universitatis Sunyatseni, 38 (1): 121～123 [8]Liu Z Y, Liang Z Q, Liu A Y, Yao Y J, Kevin D H, Yu Z N, 2002. Molecular evidence for teleomorph-anamorph connections in Cordyceps based on ITS-5.8S rDNA sequences. Mycological Research, 106:1100～1108 [9]Liu Z Y, Yao Y J, Liang Z Q, Liu A Y, David N P, Mark W C, 2001.Molecular evidence for the anamorph-teleomorph connections in Cordyceps sinensis. Mycological Research, 105:827～832 [10]梁宗琦,1983.一種國(guó)內(nèi)未見(jiàn)報(bào)道的蟲(chóng)草菌--古尼蟲(chóng)草.真菌學(xué)報(bào),2 (4):258～259 [11]梁宗琦,1985.古尼蟲(chóng)草分生孢子階段的分離和鑒定.真菌學(xué)報(bào),4(3):162～166 [12]劉愛(ài)英,梁宗琦,劉作易,1997.兩種蟲(chóng)草無(wú)性型的微循環(huán)產(chǎn)孢確證.菌物系統(tǒng),16(4):297～299 [13]J.薩姆布魯克,D.W.拉塞爾著,黃培覺(jué)等譯,2002.分子克隆實(shí)驗(yàn)指南(第三版).北京,科學(xué)出版社,461～516 [14]梁宗琦,2001.我國(guó)蟲(chóng)草屬真菌研究開(kāi)發(fā)的現(xiàn)狀及思考.食用菌學(xué)報(bào),8(2):53～62 [15]李增智,黃勃,李春如,2000.確證冬蟲(chóng)夏草無(wú)性型的分子生物學(xué)證據(jù),I.中國(guó)被毛孢與冬蟲(chóng)夏草的關(guān)系.菌物系統(tǒng),19(1):60～64 [16]趙錦,王寧,陳月琴,李泰輝,屈良鵠,1999.冬蟲(chóng)夏草無(wú)性型的分子鑒別.中山大學(xué)學(xué)報(bào)(自然科學(xué)版),38(1):12l～123 菌物學(xué)報(bào) 2005年第3期-HTM文件 No.7