雙孢蘑菇性親和性相關(guān)分子標(biāo)記的初步篩選
Primary Screening of Molecular Markers Related to Sexual Compatibility in Agaricus bisporus
詹才新 凌霞芬 楊家林
摘 要：以傳統(tǒng)的形態(tài),生理生化分析和最新的DCS-PDMA性親和性測(cè)定方法為基礎(chǔ),結(jié)合群體分離分析和RAPD技術(shù)對(duì)來源于同一雙孢蘑菇異核體菌株的12個(gè)不育同核原生質(zhì)體個(gè)體進(jìn)行分析,篩選與性親和性相關(guān)的分子標(biāo)記.研究結(jié)果表明,供試的12個(gè)不育同核原生質(zhì)體個(gè)體被分成兩大類性親和性類型,其中一類(A+)包括不育同核原生質(zhì)體個(gè)體B、 C、 D、 E、 F、 G、 H、 I、 J、 L,另一類(A-)則僅僅包括不育同核原生質(zhì)體個(gè)體K和M,同時(shí)篩選到一個(gè)與性親和性相關(guān)的分子標(biāo)記OPA161500.從而為間接地利用雙孢蘑菇本身特有的交配型作標(biāo)記來指導(dǎo)雜交育種工作和進(jìn)一步將其性親和性基因定位分離克隆奠定了堅(jiān)實(shí)的基礎(chǔ).
關(guān)鍵詞：雙孢蘑菇; DCS-PDMA方法; 群體分離分析; 性親和性相關(guān)分子標(biāo)記; 雜交育種
分類號(hào)：S464.110.36 文獻(xiàn)標(biāo)識(shí)碼：A
文章編號(hào)：1005-9873(2002)04-01-08
基金項(xiàng)目：國(guó)家自然科學(xué)基金項(xiàng)目(95-01-04)
作者簡(jiǎn)介：第一作者現(xiàn)在地址: Box 109, Laboratory of Developmental Neurobiology, the Rockefeller University,1230 York Avenue, New York, NY 10021, USA.楊家林，本文通訊作者
作者單位：詹才新（上海市農(nóng)業(yè)科學(xué)院食用菌研究所;農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開放實(shí)驗(yàn)室,上海,201106）
凌霞芬（上海市農(nóng)業(yè)科學(xué)院食用菌研究所）
楊家林（鄂州市衛(wèi)生?？茖W(xué)校,鄂州,436000）
參考文獻(xiàn)：
［1］楊新美. 中國(guó)食用菌栽培學(xué)[M]. 北京:農(nóng)業(yè)出版社,1986. 247～250.
［2］Chang S. Mushroom research and development-equality and mutual benefit[C]. In: Rose DJ (ed) Mushroom biology and mushroom products. Proceedings of the Second International Conference, University Park, Pennsylvania, Penn State Univ., 1996. 1～10.
［3］Raper CA, Miller RE, Raper JR. Genetic analysis of the life cycle of Agaricus bisporus[J]. Mycologia, 1972, 64: 1088～1117.
［4］May B, Royse DJ. Confirmation of crosses between lines of Agaricus brunnescens by isozyme analysis[J]. Experimental Mycology, 1982, 6: 283～292.
［5］Castle AJ, Horgen PA, Anderson JB. Restriction fragment length polymorphisms in the mushroom Agaricus brunnescens and Agaricus bitorquis[J]. Appl Environ Microbiol, 1987, 53:816～822.
［6］馬愛民,賀冬梅,潘迎捷. 雙孢蘑菇同核原生質(zhì)體育種技術(shù)研究[J]. 食用菌學(xué)報(bào),1995,2(3]:1～5.
［7］Zhan Caixin. Determination of sexual compatibility by DCS-PDMA method in Agaricus bisporus[R]. China-Japan International Symposium on Mycology. Wuhan, China, 1997. 50～51.
［8］Zhan Caixin, Zhu Lanbao, Yang Xinmei. Distinguishing strains of Flammulina velutipes with RAPD[J]. Journal of Huazhong Agricultural University, 1995,14 (3): 253～258.
［9］Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genome regions by using segregating populations[J]. Proc Natl Acad Sci USA, 1991, 88: 9828～9832.
［10］Paran I, Michelmore RW. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce[J]. Theor Appl Genet, 1993, 85: 985～993.
［11］Judelson HS, Spielman LJ, Shattock R. Mapping non-mendelian segregation of mating-type loci in the Oomycete, Phytophthora infestans[J]. Genetics, 1995,141:503～512.
［12］詹才新,張引芳,凌霞芬,等.中國(guó)主栽雙孢蘑菇菌株的DNA多態(tài)性[J].食用菌學(xué)報(bào),1998,5(2):1～7.
［13］詹才新,凌霞芬. 雙孢蘑菇菌落形態(tài)和產(chǎn)質(zhì)量性狀間相關(guān)性研究[J].食用菌學(xué)報(bào),1997,4(3):7～12.
［14］Kothe E. Mating-type genes for basidiomycete strain improvement in mushroom farming[J]. Appl Microbiol Biotechnol, 2001,56:602～612.
［15］Stoop JMH, Mooibroek H. Advances in genetic analysis and biotechnology of the cultivated button mushroom, Agaricus bisporus[J]. Appl Microbiol Biotechnol, 1999, 52: 474～483.
