劉競男; 王四寶; 何兵; 沈業(yè)壽; 樊美珍
安徽大學生命科學學院; 安徽農(nóng)業(yè)大學經(jīng)濟昆蟲菌物研究所; 安徽省監(jiān)獄管理局九成分局東角湖監(jiān)區(qū); 安徽大學生命科學學院; 安徽農(nóng)業(yè)大學經(jīng)濟昆蟲菌物研究所 合肥230039 ; 合肥230036 ; 望江246200 ; 合肥230039 ; 合肥230036

【中文摘要】 以菌絲體生物量為測定指標,篩選出楊樹菇液體培養(yǎng)的最適氮源為蠶蛹粉,最適碳源為可溶性淀粉;確定了楊樹菇最佳液體培養(yǎng)基配方為:蠶蛹粉4%,可溶性淀粉3%,KH2PO40.1%,MgSO4·7H2O0.3%;適宜裝液量32%,起始pH7.0,接種量15%,液體發(fā)酵周期7d。

【英文摘要】 Taking the mycelial biomass as detecting index, the optimal carbon source, nitrogen source and their optimum concentrations, the appropriate medium capacity, initial pH and mycelium harvesting time of Agrocybe aegerita in submerged culture with shake flask were studied. And the exprimental results showed that the optimal carbon and nitrogen sources were silkworm chrysalis powder and soluble starch respectively, and that the optimal compositions of liquid medium were silkworm chrysalis powder 4%, soluble starch 3%, KH_2PO_4 0.1% and MgSO_4·7H_2O 0.3%. While initial pH 7.0, medium capacity 32% (80mL/250mL flask), inoculation rate 15% and submerged culture period 7 days were proved to be the optimal submerged culture conditions for Agrocybe aegrita.

【中文關(guān)鍵詞】 楊樹菇; 液體培養(yǎng); 菌絲體生物量
【英文關(guān)鍵詞】 Agrocybe aegerita; Submerged culture; Biomass
【基金】安徽省教育廳高等學校青年教師科研基金資助項目:"珍稀食藥用菌楊樹菇液體發(fā)酵工藝及液體種速效栽培技術(shù)研究"(01080504)的部分內(nèi)容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2004年03期 【DOI】CNKI:SUN:SYJB.0.2004-03-010