SUN Xiaohong1; 2; 3; CHEN Mingjie1; PAN Yingjie11 Shanghai Edible Fungi Biotechnology and Engineering Research Center; Key Laboratory of AgriculturalGenetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China; 2 College of Life Sciences; Nanjing Agricultural University; Nanjing; Jiangsu 210095; China; 3Shanghai Fisheries University; Shanghai 200090; China

【中文摘要】 The complete sequence(from ATG to TAA) of a glyceraldehyde-3-phosphate dehydrogenase(gpd) gene isolated from Volvariella volvacea consisted of 1489 bp and contained nine introns of similar size.The full-length cDNA of gpd contained 1157 nucleotides with a predicted ORF of 1011 bp encoding for 337 amino acids and a 3'-untranslated region of 95 bp.Putative promoter elements have been identified in a 1220 bp 5'-flanking region,analysis of which revealed two TATA boxes located 80 and 559 bp upstream...更多 of the start codon,and three CAAT boxes 333,340 and 406 bp upstream of the initiating ATG.Southern blot analysis suggested that the V.volvacea genome contained only one copy of the gpd gene.Northern blot analysis revealed that gpd gene expression was not induced by cold stress,which may explain in part why this mushroom is more perishable than other mushrooms in which gpd expression increases during exposure to low temperatures.  還原

【英文摘要】 The complete sequence(from ATG to TAA) of a glyceraldehyde-3-phosphate dehydrogenase(gpd) gene isolated from Volvariella volvacea consisted of 1489 bp and contained nine introns of similar size.The full-length cDNA of gpd contained 1157 nucleotides with a predicted ORF of 1011 bp encoding for 337 amino acids and a 3'-untranslated region of 95 bp.Putative promoter elements have been identified in a 1220 bp 5'-flanking region,analysis of which revealed two TATA boxes located 80 and 559 bp upstream of the start codon,and three CAAT boxes 333,340 and 406 bp upstream of the initiating ATG.Southern blot analysis suggested that the V.volvacea genome contained only one copy of the gpd gene.Northern blot analysis revealed that gpd gene expression was not induced by cold stress,which may explain in part why this mushroom is more perishable than other mushrooms in which gpd expression increases during exposure to low temperatures.

【英文關(guān)鍵詞】 cold stress; gene expression; glyceraldehyde-3-phosphate dehydrogenase gene; regulatory elements; Volvariella volvacea
【基金】Sponsored by National Natural Science Foundation of China (No 30400301)

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2008年01期 【DOI】CNKI:SUN:SYJB.0.2008-01-007