【作者】 王玉江; 韓增華; 戴肖東; 孔祥輝; 張恩波;
【Author】 WANG Yujiang,HAN Zenghua,DAI Xiaodong,KONG Xianghui,ZHANG Enbo Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010,China
【機(jī)構(gòu)】 黑龍江省科學(xué)院微生物研究所;
【摘要】 以5個(gè)黑木耳(Auricularia auricula)栽培菌株為實(shí)驗(yàn)材料,研究了黑木耳栽培過(guò)程中4種胞外酶活性的變化。結(jié)果表明,不同菌株胞外酶活性不同,但在整個(gè)生長(zhǎng)發(fā)育過(guò)程中變化規(guī)律基本一致:即隨著黑木耳子實(shí)體的生長(zhǎng)發(fā)育,羧甲基纖維素酶(CMCase)和濾紙纖維素酶(FPase)的活性逐漸增強(qiáng),并在子實(shí)體成熟時(shí)最高,子實(shí)體采收后活性迅速下降;漆酶(LAC)、多酚氧化酶(POD)活性在菌絲生長(zhǎng)階段較高,隨著子實(shí)體的生長(zhǎng)發(fā)育酶活下降。
【Abstract】 Extracellular polyphenol oxidase(POD),carboxymethyl cellulase(CMCase),filter paper cellulase(FPase) and laccase(LAC) production by five Auricularia auricula cultivars was determined at different stages of the cultivation cycle.Although enzyme levels varied among the different cultivars,production patterns were similar in all cases.LAC and POD levels were higher during the substrate colonization phase but declined in the later stages of cultivation.Conversely,CMCase and FPase levels were relative...
【關(guān)鍵詞】 黑木耳; 栽培; 胞外酶; 漆酶; 多酚氧化酶; 羧甲基纖維素酶; 濾紙纖維素酶;
【Key words】 Auricularia auricula; cultivation; extracellular enzyme; laccase; polyphenol oxidase,carboxymethyl cellulase; filter paper cellulase;
【基金】 黑龍江省青年基金項(xiàng)目(編號(hào):QC08C58)的部分研究?jī)?nèi)容
【分類(lèi)號(hào)】S646.6