巴西蘑菇多糖誘導(dǎo)K562腫瘤細(xì)胞凋亡的研究 袁麗銘 忻茜 李興玉 摘 要：應(yīng)用流式細(xì)胞分選儀(Flow cell sorter,FCS)和透射電子顯微鏡(Transmissional electron microscope,TEM)等技術(shù)觀察了巴西蘑菇(Agaricus blazei)多糖誘導(dǎo)K562腫瘤細(xì)胞凋亡的試驗(yàn)研究.試驗(yàn)發(fā)現(xiàn),流式細(xì)胞儀可檢測到經(jīng)巴西蘑菇多糖誘導(dǎo)K562腫瘤96h后產(chǎn)生明顯的亞二倍體峰;電鏡下觀察到K562腫瘤細(xì)胞核固縮,核內(nèi)染色質(zhì)濃縮、凝聚,緊靠在細(xì)胞核膜邊沿,形成新月狀或環(huán)狀結(jié)構(gòu)等典型的凋亡細(xì)胞的形態(tài)變化及凋亡小體.結(jié)果表明,巴西蘑菇多糖具有誘導(dǎo)K562腫瘤細(xì)胞凋亡的作用. 關(guān)鍵詞：巴西蘑菇多糖;誘導(dǎo);K562腫瘤細(xì)胞;細(xì)胞凋亡 分類號：S646.110;R151.3 文獻(xiàn)標(biāo)識碼：A 文章編號：1005-9873(2005)03-0038-05 The Apoptosis of K562 Tumor Cells Induced by Polysaccharide Extracted from Agaricus blazei YUAN Li-ming XIN Xi LI Xing-yu 基金項(xiàng)目：上海市高等院?？茖W(xué)技術(shù)發(fā)展基金項(xiàng)目"巴西蘑菇DNA反義核酸抗腫瘤的實(shí)驗(yàn)研究"(編號:02DK09)的部分研究內(nèi)容 作者簡介：袁麗銘(1980-),女,上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院在讀碩士研究生,主要從事動(dòng)物細(xì)胞與分子生物學(xué)方面的研究,發(fā)表主筆論文2篇. 作者單位：袁麗銘（上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院,上海,200234） 忻茜（上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院,上海,200234） 李興玉（上海師范大學(xué)生命與環(huán)境科學(xué)學(xué)院,上海,200234） 參考文獻(xiàn)： [1]張秀文,王鏡,王菊勇,等.姬松茸對馬利蘭導(dǎo)致骨衰竭小鼠的治療作用[J].蘭州醫(yī)學(xué)院學(xué)報(bào),1998,24(4):14. [2]王立榮,王鏡.姬松茸治療慢性肝炎的臨床研究[J].白求恩醫(yī)科大學(xué)學(xué)報(bào),1995,211(4):14. [3]Mizuno T. Food reviews international, antitumor-active heteroglycans from Niohshimeji mushroom,Tricholoma giganteum [J]. Biosci Biotechnol Biochem, 1995,59(4) :568～571. [4]近藤賢(劉雪卿譯).巴西蘑菇與癌癥[M].臺灣:正義出版社,1998. [5]Ito H, Shimura K, Kawade M, et al. Antitumor effects of a new polysaccharide-protein complex (ATOM)prepared from Agaricus blazei (Iwade strain 101) "Himematsutake" and its mechanisms in tumor-bearing mice[J]. Anticancer Res, 1997,17(1A) :277～284. [6]Cohen JJ. Programmed cell death in the immune system[J]. Adv. Immunal, 1991,50:55～85. [7]Loten J and Sachs L. A mutant p53 antagonizes the deregulated c-myc-mediated enhancement of apoptosis and decrease in leukemogenicity[ J ]. USA: Proc. Natl. Acad. Sci, 1995, (21): 9672～9676. [8]Wylie AH. Cell Death, the significance of apoptosis[J]. Int. Rev. Cytology, 1980,168:251. [9]Henkart PA. Apoptosis: what is the sring[J]? J. Immunal, 1995,154(10):4905～4908. [10]Scholze Sthoff, Droge KW, Krammer PH. Cell nucleus and DNA fragmentation are not required for apoptosis [J]. J. Cell Biol, 1994,127(1) :15～20. [11]Telford WG, King LE, Fraker PJ. Rapid quantitation of apoptosis in pureand hererogeneous cell populations using flow cytometry[J]. J. Innumo. Meth, 1994, (172): 1～16. [12]Mccloskey TW, Oyaizu N, Loronesi M, et al. Use of a flow cytometric assay to quamitate apoptosis in human lymphocytes[J]. Clin Immunol. Immunopathal, 1994,71( 1 ): 14～18. 食用菌學(xué)報(bào) 2005年第3期-HTM文件 No.7
