阿魏蘑多糖理化性質(zhì)及免疫活性研究

甘勇　 呂作舟

摘　要：以阿魏蘑PleurotusferulaeLanzi子實體和菌絲體為試驗材料，采用水浸法提取阿魏蘑多糖，分別得到子實體粗多糖A和菌絲體粗多糖B。將A經(jīng)Sevag法去蛋白、透析、CTAB絡(luò)合、乙醇沉淀、NaCl溶液溶解、透析，得到多糖A1。紫外光譜分析鑒定多糖A1為均一組分。苯酚-硫酸法測得多糖A1糖含量為82.9%。凝膠滲透色譜法測得多糖A1數(shù)均分子量Mn=141088，重均分子量Mw=142897。氣相色譜分析多糖A1單糖組成及其摩爾比為Xyl∶Gla∶Glc=1∶1.102∶2.899。巨噬細(xì)胞吞噬作用試驗、遲發(fā)型變態(tài)反應(yīng)試驗、白細(xì)胞介素-2（IL-2）的誘生與檢測試驗測得粗多糖A、粗多糖B具有免疫活性。

關(guān)鍵詞：阿魏蘑，多糖，理化性質(zhì)，免疫活性?オ?

分類號：Q939.96　文獻(xiàn)標(biāo)識碼：A

文章編號：1007-3515（2001）02-0228-0232

STUDIES ON PHYSICAL AND CHEMICAL PROPERTIES AND IMMUNOLOGICAL ACTIVITIES OF POLYSACCHARIDES FROM PLEUROTUS FERULAE??

GAN Yong（The Laboratory of Applied Mycology, HuaZhong Agricultural University, Wuhan 430070）　

LU Zuo-Zhou（The Laboratory of Applied Mycology, HuaZhong Agricultural University, Wuhan 430070）　

Abstract：Crude polysaccharides A and B were extracted separately from the fruiting body and cultured mycelium of Pleurotus ferulae Lanzi with water-extracted method. For obtaining polysaccharide A1,protein was removed from crude polysaccharide A with Sevag method, dialysed with water, precipitated with CTAB and alcohol, dissolved with NaCl solution and then dialysed again . Identification of ultraviolet spectrum showed that the polysaccharide A1 was a homogeneous composition, and the purity of polysaccharide was 82.9% ,judging by the Phenol-H2SO4 method analysis. GPC (gel permeation chromatography)detection showed that the number average molecular weight of polysaccharide A1 was 141,088 and the weight average molecular weight of polysaccharide A1 was 142,897. GC (gas chromatography) analysis indicated the monosaccharides' composition and molar ratio of polysaccharide A1 were:Xyl∶Gla∶Glc=1∶1.102∶2.899.It was proved the crude polysaccharide A and B had immunological activities according to the experiments of macrophage phagocytosis, delayed type hypersensitivity and induction and detection of IL-2.

Keywords：Pleurotus ferulae, polysaccharide, physical and chemical property, immunological activity.

基金項目：湖北省重點科技計劃（952P1007）資助項目

作者單位：甘勇（華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究室武漢 430070）　

　　　　　呂作舟（華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究室武漢 430070）　

　　　　　甘勇（山東魯南制藥集團(tuán)，臨浙 236000）　

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