蟲草多糖對日本沼蝦免疫機(jī)能的影響

昌鳴先　 陳孝煊　 吳志新　 胡先勤　

摘　要：在飼料中添加1‰的蟲草多糖，以口服形式對日本沼蝦進(jìn)行免疫。通過連續(xù)測定日本沼蝦血淋巴吞噬活性，血清中抗菌活力、溶菌活力以及酚氧化酶活力，研究蟲草多糖對日本沼蝦免疫機(jī)能的作用。結(jié)果表明：除個別情況外，蟲草組的吞噬百分比和吞噬指數(shù)、抗菌活力、溶菌活力以及酚氧化酶活力，均高于對照組。因此,投喂蟲草多糖能夠明顯地增強(qiáng)日本沼蝦的免疫防御能力。

關(guān)鍵詞：免疫機(jī)能；蟲草多糖；日本沼蝦

分類號：S 963．73+6；S 945.4+9；S 942．5

The Effect of Cordyceps Polysaccharide on Macrobrachium nipponense Immune Function

Chang Mingxian（Huazhong Agricultural University, Wuhan 430070, China）　

Chen Xiaoxuan（Huazhong Agricultural University, Wuhan 430070, China）　

Wu Zhixin（Huazhong Agricultural University, Wuhan 430070, China）　

Hu Xianqin（Huazhong Agricultural University, Wuhan 430070, China）　

Abstract：Cordyceps polysaccharide, a product bought from med icine shops, with th e characteristics similar to those of β-glucan, was used as a food additive to test its immune effect. Macrobrachbium nipponense with a body we ight of 2.0～2.5 g were collected from the Dadongmen Aquaculture Market on Sept em ber 12, 2000，and nursed. Water temperature varied from 29℃　to　14℃ （n atural temperature），the water was aerated and changed once a day（1/2～2/3 volume of water was renewed each time）. 　　The experiment of phenoloxidase （PO） test was designed as the same as those of bacteriolytic（Ul）and antibacterial （Ua）activity tests. Blood samples w ere c ollected from the haemocoel of 10～15 shrimps to measure PO, Ul, Ua activity on 0, 1, 4, 7, 14, 21 day. The phagocytosis was still measured after stopping the polysaccharide's feeding. 　　The results showed that PO, Ua, Ul and phagocytosis of the test group are si gnif icantly higher than those of the controll group, indicating the effectiveness of cordceps polysaccharide on enhancing PO, Ua, Ul and phagocytosis of M. ni pponen se，leading to an increase in the immunnological activities. Further studies a re needed to identify the mechanism of cordceps polysaccharide in immune effect.

Keywords：immune function, Cordyceps polysaccharide, Macrob rachium nipponense

基金項目：武漢市重點科技項目

作者簡介：昌鳴先，女，1972年生，華中農(nóng)業(yè)大學(xué)水產(chǎn)學(xué)院碩士生．武漢 430070

作者單位：昌鳴先（華中農(nóng)業(yè)大學(xué)水產(chǎn)學(xué)院，武漢 430070）　

　　　　　陳孝煊（華中農(nóng)業(yè)大學(xué)水產(chǎn)學(xué)院，武漢 430070）　

　　　　　吳志新（華中農(nóng)業(yè)大學(xué)水產(chǎn)學(xué)院，武漢 430070）　

　　　　　胡先勤（華中農(nóng)業(yè)大學(xué)水產(chǎn)學(xué)院，武漢 430070）　

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華中農(nóng)業(yè)大學(xué)學(xué)報

JOURNAL OF HUAZHONG AGRICULTURAL UNIVERSITY

2001 Vol.20 No.3 P.275-278