突臍孢屬Brnl基因核苷酸序列比較及系統(tǒng)發(fā)育研究

SEQUENCE COMPARISON AND PHYLOGENETIC ANALYSIS OF BRN1 GENES FROM EXSEROHIL UM SPECIES

孫廣宇　張雅梅　張榮　

摘　要：對所有供試突臍孢菌株的Brnl基因(1,3,8-三羥基萘還原酶基因)擴增均獲得PCR產(chǎn)物.序列比較表明:在種內各菌株間沒有核苷酸序列長度變化,存在核苷酸序列簡單代換;在種間核苷酸序列長度有變化,核苷酸的缺失或插入發(fā)生在內含子區(qū);所有菌株編碼區(qū)核苷酸序列長度相同;在種內水平氨基酸序列沒有差別,顯示出高度的保守性.利用最大簡約法(Maximum Parsimony)和鄰近結合法(Neighbor-joining)構建系統(tǒng)發(fā)育樹,兩個系統(tǒng)發(fā)育樹的拓撲結構相似,不同種在不同的分支上.Brn1基因適合突臍孢屬種級水平的分子系統(tǒng)學研究.

關鍵詞：系統(tǒng)發(fā)育樹;離蠕孢屬;序列分析

分類號：Q939.97 　文獻標識碼：A

文章編號：1672-6472(2004)04-0480-0486

基金項目：國家自然科學基金資助項目(項目編號:30170004)

作者簡介：孫廣宇,通訊作者 E-mail:sunguang@public.xa.sn.cn

作者單位：孫廣宇（西北農(nóng)林科技大學植保學院,陜西,楊凌,712100）　

　　　　　張雅梅（西北農(nóng)林科技大學植保學院,陜西,楊凌,712100）　

　　　　　張榮（西北農(nóng)林科技大學植保學院,陜西,楊凌,712100）　

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收稿日期：2004年3月8日

修稿日期：2004年5月20日

出版日期：2004年11月22日

菌物學報

MYCOSYSTEMA

2004 Vol.23 No.4 P.480-486