李向敏; 謝意珍; 潘鴻輝; 簡(jiǎn)偉明; 羅彪; 李森柱; 張智
廣東粵微食用菌技術(shù)有限公司; 廣東省微生物研究所

【中文摘要】 利用多種分析檢測(cè)手段和腫瘤細(xì)胞實(shí)驗(yàn),研究了靈芝孢子油的質(zhì)量檢測(cè)參數(shù)與其抗腫瘤細(xì)胞活性的關(guān)系,為靈芝孢子油質(zhì)量檢測(cè)標(biāo)準(zhǔn)的建立提供實(shí)驗(yàn)參考依據(jù)。取6種不同的經(jīng)CO2超臨界提取的靈芝孢子油樣品,用KOH標(biāo)準(zhǔn)滴定法測(cè)酸價(jià)、KI-I2法檢測(cè)過(guò)氧化值、紫外-可見(jiàn)光分光光度法測(cè)總?cè)啤Ｍ瑫r(shí)利用腫瘤細(xì)胞檢測(cè)6種樣品對(duì)人惡性乳腺癌細(xì)胞(MT-1)和淋巴瘤細(xì)胞(Jurka)t的抑制作用。結(jié)果表明,樣品的酸價(jià)與總?cè)坪亢推淇鼓[瘤效果呈正相關(guān),6種樣品的過(guò)氧化值變化不大。高酸價(jià)樣品1,其總?cè)坪?16.00%)較高,在1μL·mL-1濃度抑制時(shí),能夠抑制絕大部分腫瘤細(xì)胞的生長(zhǎng),具有較高的抗腫瘤活性。

【英文摘要】 The relationship of Ganoderma lucidum spore oil quality testing standards and its anti-tumor cell activity to provide parameters for the establishment of testing standards were studied. Ganoderma lucidum spore oil was extracted by CO2 supercritical fluid, which had six different samples. The acid value, the peroxide value, total triterpenoids were determined. The inhibition effect of Ganoderma lucidum spore oil on human malignant breast cancer cells (MT-1) and human leukemia cells (Ju-rkat) were studied by cell culture method. The results showed that anti-tumor effect was positively correlated with acid value and the total level of triterpenoids.

【中文關(guān)鍵詞】 靈芝孢子油; 三萜; 抗腫瘤細(xì)胞活性
【英文關(guān)鍵詞】 Ganoderma lucidum spore oil; Total triterpenoids; Anti-tumor cell activity
【基金】廣州開(kāi)發(fā)區(qū)經(jīng)濟(jì)區(qū)科技資助項(xiàng)目(2009Q-P143);; 廣東省科技廳農(nóng)業(yè)攻關(guān)資助項(xiàng)目(2009B080701065)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年02期 【DOI】CNKI:SUN:ZSYJ.0.2010-02-019