陳體強(qiáng); 翁啟勇; 黃大斌
福建省農(nóng)科院植保研究所!福州350013; 福建省農(nóng)科院植保研究所!福州350013; 福建省農(nóng)科院植保研究所!福州350013
【中文摘要】 應(yīng)用梯度濃度聚丙烯酰胺凝膠電泳法(GC-PAGE)對(duì)金針菇白色菌株和普通菌株的菌體可溶性蛋白質(zhì)進(jìn)行分析比較.結(jié)果表明,可溶性蛋白質(zhì)譜帶分布在凝膠濃度6.4%~23.6%范圍內(nèi),具有19~23條,其中15條為金針菇共同譜帶,普通菌株的蛋白質(zhì)譜帶與白色菌株差異明顯;白色菌株間譜帶相似程度較大,相似系數(shù)(SC)為0.786~0.818;推廣栽培品種8909(B)存在其特征性譜帶.
【英文摘要】 The soluble mycoprotein of white strains and norraul yellow strain of F. velutipes was analyzed by gradient concentration polyacrylamidc gel oleetrophoresis (GC-PAGE). The results showed that 19-23 soluble mycoprotein band were distributed in gel concentration ranging from 6.4% to 23.6H, in which 15 bands were common bands. The band of normal strains was significantly different from that of white strains. The bands were similar between white strains, SC=0.786-0.818. While the popularized strain 8909(B) was provided with characteristic bands.
【中文關(guān)鍵詞】 金針菇; 白色菌株; 可溶性蛋白質(zhì); 電泳圖譜
【英文關(guān)鍵詞】 Flammulina velutipes; White strain; Soluble mycoprotein; ELectrophoresis chromatogram
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1995年02期 【DOI】CNKI:SUN:SYJB.0.1995-02-011