為了充分開發(fā)利用靈芝資源，本研究對采自西藏墨脫縣的一份野生靈芝子實體標本進行了分離純化培養(yǎng)，并通過形態(tài)學觀察和分子生物學分析鑒定為有柄靈芝Ganoderma gibbosum。將此菌株作為試驗菌株，從碳源、氮源、溫度、pH這4個因素進行生物學特性研究，從中選擇3個較優(yōu)水平進行正交試驗。結(jié)果表明，有柄靈芝菌絲生長的最佳碳源為果糖，氮源為酵母，最適溫度為25 ℃，適宜pH為7.0。馴化出芝栽培基質(zhì)配方選用雜木屑58%、玉米芯20%、麥麩20%、石灰1%、石膏1%，40 d左右菌絲滿袋，覆土一周后出現(xiàn)原基，50 d左右子實體成熟。初步成分分析表明，有柄靈芝粗多糖、氨基酸含量分別為1.5%和10.8%。本試驗成功對野生有柄靈芝進行了人工馴化栽培，為有柄靈芝作為新的藥用菌進行開發(fā)利用提供了參考。

A wild strain of Ganoderma from Medog, Xizang, was identified as Ganoderma gibbosum, based on morphological observation and molecular biological analysis. Its biological characteristics and domestic cultivation were studied. Single-factor tests were conducted for optimizing carbon and nitrogen sources, temperature and pH under solid cultivation conditions. Orthogonal experiment of three optimal levels selected from the four single-factor tests showed that the optimal carbon source and nitrogen source for mycelial growth of G. gibbosum were fructose and yeast, respectively. The optimum temperature was 25 ℃, and the suitable pH was 7.0. The fruiting bodies of G. gibbosum could be formed on the substrate formulated by 58% angiosperm wood chips, 20% corncob, 20% bran, 1% lime, and 1% gypsum. The hyphae fully colonized the substrate after cultivation for 40 days. The primordium began to form in one week after casing, and the fruiting body matured in about 50 days. Preliminary analysis of functional components indicated that polysaccharide and amino acid contents of G. gibbosum were 1.5% and 10.8%, respectively. G. gibbosum might have a new potential medicinal value in future applications.