沈天峰; 王朝江; 羅信昌; 池惠榮
河南省科學(xué)院生物研究所; 河北省農(nóng)科院農(nóng)業(yè)物理研究所; 華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究室; 河北省農(nóng)科院農(nóng)業(yè)物理研究所 鄭州450008 ; 石家莊050000 ; 武漢430070 ; 石家莊050000
【中文摘要】 應(yīng)用RAPD技術(shù)對(duì)木耳屬四個(gè)野生菌株進(jìn)行了DNA指紋圖譜分析。綜合 10個(gè)隨機(jī)引物的RAPD聚類分析結(jié)果表明 ,供試的四個(gè)野生菌株與毛木耳 (Auriculariapolytricha)歸為一類 ,四個(gè)野生菌株間存在一定的遺傳差異 ,不是同一菌株。本研究揭示了木耳屬四個(gè)野生菌株的類源關(guān)系及DNA同質(zhì)性 ,為進(jìn)一步界定其系統(tǒng)分類地位提供了較為重要的科學(xué)依據(jù)。
【英文摘要】 DNA fingerprints of four wild strains of Auricularia were analyzed by RAPD technique. These four wild strains were grouped into Auricularia polytricha based on the result of RAPD cluster analysis with ten random primers. But they were four distinct strains because there were certain genetic differences among them. The result revealed the phylogenetic relationship and the DNA homogeneity among these four wild strains of Auricularia. And it also provided more important scientific basis for delimiting their taxonomic position.
【中文關(guān)鍵詞】 木耳屬; 野生菌株; RAPD
【英文關(guān)鍵詞】 Auricularia; Wild strain; RAPD
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2001年04期 【DOI】CNKI:SUN:SYJB.0.2001-04-000