鮑氏層孔菌培養(yǎng)條件的研究 ARTIFICAL CULTURE OF PHELLINUS BAUMII 劉春靜 張曉陽 戴玉成 魏玉蓮 摘 要:本項研究對分離于野外的鮑氏層孔菌從不同的溫度、pH值以及不同的碳源和氮源的營養(yǎng)成分三方面進(jìn)行室內(nèi)培養(yǎng),其結(jié)果顯示鮑氏層孔菌營養(yǎng)菌絲生長的最適溫度為28C;最佳碳源為甘露醇和葡萄糖;最佳氮源為蛋白胨,其次為牛肉膏;而pH值在6.0~8.0的范圍內(nèi)變化對其菌絲體生長影響不大.這些結(jié)果為將來大規(guī)模人工培植鮑氏層孔菌提供了很好的指導(dǎo)作用. 關(guān)鍵詞:室內(nèi)培養(yǎng);最適溫度;最佳碳源;最佳氮源 分類號:Q939.96 文獻(xiàn)標(biāo)識碼:A 文章編號:1672-6472(2005)02-0306-0309 基金項目:國家杰出青年科學(xué)基金資助(30425042) 作者簡介:魏玉蓮,通訊作者 作者單位:劉春靜(鐵嶺市林業(yè)科學(xué)研究院,鐵嶺,112000) 張曉陽(中國科學(xué)院沈陽應(yīng)用生態(tài)研究所,沈陽,110016) 戴玉成(中國科學(xué)院沈陽應(yīng)用生態(tài)研究所,沈陽,110016) 魏玉蓮(中國科學(xué)院沈陽應(yīng)用生態(tài)研究所,沈陽,110016) 參考文獻(xiàn): [1]Chung KS, Kim SS, Rhyu, CH, Man WH, 1991. An investigation on the antitumor constituents ofPhellinus linteus. Korean J Mycol,19:361 [2]Han SB, Lee CW, Jeon YJ, Hong ND, Yoo ID, Yang KH, Kim HM, 1999. The inhibitory effect of polysaccharide isolated from Phellinus linteus on tumor growth and metastasis. Immunopharmacology , 41: 157~164 [3]Ikekawa T, Nakanishi M, Uehara N, Chihara, G, Fukuoka, F, 1968. Antitumor action of some basidiomycetes, especially Phellinus linteus. Gann, 59:155~157 [4]Kim YS, Park KS, Park HK, Kim SW, 1994. Compositional sugar analysis of antitumor polysaccharides by high performance liquid chromatography and gas chromatography. Arch Phar Res, 17:337~342 [5]Kong DH, Hong ND, Han SB, 1996. Stimulation of humeral and cell mediated immunity by polysaccharide from mushroom Phellinus linteus. Intern J Immunopharmacol, 18:295 [6]Naruse S, Takeda S, Ito H, Jujii K, Terada Y, Shimura K, Sugiura M, Miyazaki T, 1974. Studies on antitumor activity of basidiomycetes:2. Antitumor effects of polysaccharides prepared from cultured basidiomycetes. Mie Meal J, 23:207~230 [7]Oho GT, Han SB, 1993. Immunostimulation activity ofPhellinus linteus extracts to B-lymphocyte. Arch Phar Res, 15:379~381 [8]Sasaki T, Arai Y, Ikekawa T, Chihara G, Fukuoka F, 1971. Anfitumor polysaccharides from some polyporaceae, Ganoderma applanatum (Pets.) Pat. and Phellinus linteus (Berk. & Curt.) Tang. Chem Pharm Bull, 19:821 菌物學(xué)報 MYCOSYSTEMA 2005 Vol.24 No.2 P.306-309