【作者】劉春滟; 李南羿; 張玉瓊
【Author】LIU Chunyan1; LI Nanyi2; ZHANG Yuqiong11College of Life Science; Anhui Agricultural University; Hefei; Anhui 230036; China; 2 College of Agriculture and Food; Zhejiang Forestry University; Lin’an; Zhejiang 311300; China
【作者機(jī)構(gòu)】安徽農(nóng)業(yè)大學(xué)生命科學(xué)院; 浙江林學(xué)院農(nóng)業(yè)與食品學(xué)院
【摘要】 從NCBI中下載了12 184條香菇(Lentinula edodes)表達(dá)序列標(biāo)簽(expressed sequence tag,EST)序列,處理后得到全長3 681 177 bp的4 684個Unigene。在其中共發(fā)掘出142個簡單重復(fù)序列(simple sequencerepeat,SSR),分布于120條EST中,分布頻率為2.99%(平均分布距離:25.924 kb)。其中,單、二、三核苷酸重復(fù)是主要的重復(fù)類型,A/T,AG/CT,ACG/CTG是單、二、三核苷酸的主要重復(fù)基序,分別占所有EST-SSR的23.23%、21.83%和10.56%。利用引物設(shè)計(jì)軟件PrimerPremier5.0設(shè)計(jì)了40對引物,并利用6%變性聚丙烯酰胺凝膠在18個香菇品種中進(jìn)行檢測。其中,22對引物為多態(tài)性引物,多態(tài)率為55%。應(yīng)用NTSYS軟件的聚類方法分析表明,18份材料遺傳相似系數(shù)范圍為0.3750.984,與前人研究結(jié)果相符。
【Abstract】 A total of 12 184 Lentinula edodes expressed sequence tags(ESTs) were downloaded from NCBI and shown to be associated with 4 684 Unigenes comprising 3 681 177 bp in length.A MISA search revealed a total of 142 simple sequence repeats(SSRs) with a frequency of 2.99% and an average distance distribution of 25.9 kb.The most abundant mononucleotide,dinuclotide and trinucleotide repeats were A/T,AG/CT and ACG/CTG,accounting for 23.23%,21.83% and 10.56% of each type,respectively.Forty EST-SSR primer pairs were designed by PrimerPremier 5.0,22 of which revealed polymorphic bands in the DNA of 18 L.edodes strains.NTSYS-computed similarity coefficients for the 18 strains ranged between 0.3750.984,which was approximately in accordance with their diversity based on previous research.
【關(guān)鍵詞】 香菇; EST序列; SSR標(biāo)記; 多態(tài)性; 遺傳多樣性
【Key words】 Lentinula edodes; expressed sequence tag(EST); simple sequence repeat(SSR) marker; polymorphism; genetic diversity
【基金】上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室開放課題“香菇EST-SSR標(biāo)記的開發(fā)、鑒定及其在種質(zhì)資源多樣性分析中的應(yīng)用”(編號:shagb2009-03)的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-001
【分類號】S646.12