【作者】 賈培培; 盧偉東; 郭立忠; 姜輝; 王勝蕊;
【Author】 JIA Peipei,LU Weidong,GUO Lizhong,JIANG Hui,WANG Shengrui Shandong Province Applied Mycology Key Laboratory,Qingdao Agricultural University,Qingdao,Shandong 266109,China
【機(jī)構(gòu)】 青島農(nóng)業(yè)大學(xué)應(yīng)用真菌省級(jí)重點(diǎn)實(shí)驗(yàn)室;
【摘要】 從野生的繡球菌(Sparassis crispa)子實(shí)體中分離純化繡球菌菌株,通過平板試驗(yàn)、液體發(fā)酵試驗(yàn)篩選繡球菌菌絲生長(zhǎng)的最佳固體和液體培養(yǎng)基,并用預(yù)處理過的松木屑等為材料栽培繡球菌,結(jié)果表明:最佳培養(yǎng)基分別為M6(啤酒酵母1.0%、蜂蜜粉0.3%、蛋白胨0.1%、大豆粉0.1%、KH2PO40.1%、瓊脂2%p、H 4.5)、L4(M6去掉瓊脂);菌絲在2325℃條件下培養(yǎng),約2個(gè)月顯蕾,菇蕾在1215℃培養(yǎng),約4個(gè)月得到成熟的子實(shí)體,生物轉(zhuǎn)化率約57%。
【Abstract】 A strain of Sparassis crispa was isolated and purified from a fruit body collected in the wild.Fungal mycelium grew optimally on a solid medium(M6) consisting of(w/w) brewers yeast 1.0%、honey powder 0.3%、peptone 0.1%、soybean meal 0.1%、KH2PO4 0.1%、agar 2%、pH 4.5,and on L4 liquid medium(M6 medium without added agar).When cultivated on pretreated sawdust,the strain grew optimally at 2325 ℃ and formed buds.After cultivation at 1215 ℃ for a further four months,mature fruit bodie...
【關(guān)鍵詞】 繡球菌; 固體培養(yǎng)基; 液體培養(yǎng)基; 培養(yǎng)料; 人工栽培;
【Key words】 Sparassis crispa; solid medium; liquid medium; compost; artificial cultivation;
【基金】 山東省良種產(chǎn)業(yè)化項(xiàng)目“山東設(shè)施名菜及食用菌新品種選育”(編號(hào):NYLZ2009-06)的部分研究?jī)?nèi)容
【分類號(hào)】S646